The Ki-32 saw extensive war service in the Second Sino-Japanese War, equipping the 3rd, 6th, 10th, 35th, 45th, 65th and 75th Sentai. It also saw combat during the Battle of Nomonhan against the Soviet Union in 1938-1939. Its last combat action was bombing Commonwealth forces during the Japanese Invasion of Hong Kong.
Ki-32s during World War II were also supplied to the Manchukuo Air Force to replace their obsolescent Kawasaki Type 88/KDA-2 light bombers; they were the main bomber of that service through the conflict.
After their withdrawal from front-line service in 1942 the Ki-32s were used in a training role.